It facilitates the detection of parasite eggs that infected subjects pass in their feces. The sensitivity of the kato katz technique for different infection intensities was calculated using eq 4 and is plotted in fig 3. A more detailed description of the study sites and data collection procedures. A comparison of the sensitivity and fecal egg counts of. M a n u a l o f b a s i c techniques for a health laboratory 2 n d e d i t i o n manual of basic techniques for a heal th labora y 2nd edition 9 789241 545303 who isbn 9241545305. The katokatz technique is a widespread tool in intestinal helminth. Kato havas available for download and read online in other formats. Our dosefinding study revealed an excellent tolerability profile of oxantel pamoate in children infected with t trichiura. Katokatz techniquecellophane faecal thick smear plos. Comparison of the katokatz, wet mount, and formolether. Performance of katokatz and sedimentation techniques in the di agnosis of schistosoma. Fecal samples from 194 individuals living in an area of brazil endemic for schistosoma mansoni were analyzed by a polymerase chain reaction pcr and the kato katz parasitologic examination. Comparison of polymerase chain reaction and the kato katz technique for diagnosing infection with schistosoma mansoni.
The katokatz technique is the most widely used method detecting soil transmitted helminth eggs in faecal samples. The prevalences of intestinal parasites using the single wet mount, fec, and katokatz thick smear techniques were 38. Who has recommended kato katz technique in areas with moderate to high transmission. The present study compares the kato katz technique with three other coproparasitological methods for detecting eggs of amphimerus in stools, as well as determines the prevalence of infection in chachi residents in a tropical rain forest area in the northwest coast of ecuador. Yet, studies comparing diagnostic methods for the enumeration of eggs in stool are few. The kato katz technique is relatively simple and inexpensive and sufficiently sensitive to detect moderate and heavy s. In this study we evaluated three methods used in the diagnosis of schistosoma mansoni infection. We assessed the comparability of the average amount of faeces generated by three kato katz templates included in test kits from two different providers. For the love of physics walter lewin may 16, 2011 duration. Assessing stool quantities generated by three specific katokatz. The flotac technique shows a higher sensitivity for the diagnosis of lightintensity soiltransmitted helminth infections but is reported to be more complex and expensive.
Pdf the katokatz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials pertaining. Nine hundred kato katz thick smear preparations were done. Pathology and animal health, university of naples federico ii, naples, italy. The aim of this study was to evaluate the operational characteristics sensitivity and negative predictive value npv of wet mount, formolether concentration fec, and kato katz techniques for the determination of intestinal parasitic infections. More detailed instructions can be found in laboratory manuals and in the who manual on preventive chemotherapy in human helminthiasis. For the diagnosis of common sths, the world health organization who recommends the kato katz technique 7,8, which is a relatively straightforward method for determining the prevalence and intensity of such infections. Accuracy, diagnosis, infection intensity, kato katz technique.
The kato katz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials pertaining to intestinal schistosomiasis and soiltransmitted helminthiasis. The sensitivity of the thick smear technique was higher than that of the kato katz method for the diagnosis of all helminths except schistosoma mansoni keywords. The diagnostic performance of the kato katz kk technique and the pointofcare circulating cathodic antigen poccca test in detecting s. The kato technique is now most commonly used for detecting schistosome eggs. A study was carried out with 425 children aged 014 yr residing in bolifamba, cameroon, to investigate the effect of plasmodium falciparum malaria and intestinal helminth coinfection on anemia and to identify significant predictors of anemia in the community. A collection of anecdotes and reflections on language and language learning, it frequently recalls the pragmatism of similar texts by polyglot linguists such as bloomfield outline guide. Novel approaches to the diagnosis of strongyloides. Comparison of the thick smear and kato katz techniques for diagnosis of intestinal helminth infections. It is a direct concentration quantitative technique with high specificity reaching 100% 15. Evaluation of immunological, parasitological and molecular. A comparison of katokatz technique to three other methods for. The influence of sampling effort and the performance of.
Pdf comparison of polymerase chain reaction and the kato. However, a range of methods, including direct microscopy, formolether concentration fec, mcmaster, flotac, and miniflotac, are avialable. For hookworm the dependence on infection intensity is weak, e. To compare the relative performance of the kato katz or other qualitative coprological techniques and the mcmaster egg counting technique. Estimating sensitivity of the katokatz technique for the diagnosis of. However, sensitivity rose to more than 95% for a setting with a mean infection intensity of over 300 epg. Who has recommended kato katz technique in areas with moderate to high transmission rates of soil transmitted helminths i.
Pdf the present study compared the katokatz thick smear and formol ether sedimentation techniques. Later multiply by the appropriate number see inletinformation of the kato set to give the number of the eggs per gram faeces. However, the sensitivity of the technique is low, particularly for the detection of lightintensity helminth infections. Should this approach be corroborated by further studies, sts could become a good and inexpensive method to improve the rate of s.
Sterlitech, an industry leader in unique micro and submicron filtration products, announced today that its katokatz technique diagnostic kit is now available for immediate use in the field. Coproscopic techniques examination of faecal material by kato katz kk technique is the most widely used assay for the diagnosis of intestinal schistosomiasis. Assessing stool quantities generated by three specific. Albonico, m, sm ame, jozef vercruysse, and bruno levecke. Transactions of the royal society of tropical medicine and hygiene 106 3. Estimating sensitivity of the katokatz technique for the.
Each kit includes materials to conduct 500 sample tests according to the current who standard of fecal sample sizes of 41. Determining soiltransmitted helminth infection status and. Diagnostic performance of kato katz technique and pointof. Intestinal schistosomiasis and soiltransmitted helminthiasis. Pdf estimating sensitivity of the katokatz technique for the. Estimating the sensitivity and specificity of kato katz stool examination technique for detection of hookworms, ascaris lumbricoides and trichuris trichiura infections in humans in the absence of a gold standard v oguoma, c ekwunife. Fecal examination is the standard diagnostic method to diagnose most parasitic infections of the gastrointestinal tract, which include clonorchis sinensis infection. Comparison between merthiolateiodineformalin and kato. Quality control in the diagnosis of trichuris trichiura and ascaris. Performance of kato katz and sedimentation techniques in the di agnosis of schistosoma mansoni according to the intens ity of in fecti on. The katokatz technique is the most widely used method detecting soiltransmitted helminth eggs in faecal samples.
Comparison of the thick smear and katokatz techniques for. The kato technique also called the katokatz technique is a laboratory method for preparing human stool samples prior to searching for parasite eggs. This study compared the efficiency of kato katz thick smear and thick smear techniques for the diagnosis of intestinal helminths. The kato technique also called the kato katz technique is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Diagnosis of intestinal parasites through examination of fresh faecal samples is hampered by its unpleasantness and the urgent need to detect all parasitic forms.
The cellophane thick smear kato or kato katz method and formalinether concentration fe techniques are two such modalities commonly used for its diagnosis. Infection with sth can be readily diagnosed by detection of helminth eggs in stool samples using microscopic techniques. The katokatz technique is recommended for the diagnosis of helminth infections in. Accuracy, diagnosis, infection intensity, katokatz technique. The most widely used approach is the kato katz technique, which is also recommended by the world health organization. Easy to use fieldbased test kit for the kato katz technique in a lightweight and portable box. Blood was collected by finger prick to determine malaria parasitemia and packed cell volume pcv. Efficacy and safety of oxantel pamoate in schoolaged. A total of 354 faecal specimens were collected from students in northwest ethiopia and screened with kato katz, wet mount. The kato katz technique, despite its known limitations, is routinely used and remains the standard diagnostic technique for schistosomiasis. Virgin coconut oil as an alternative clearing agent used. Pdf comparison of a polymerase chain reaction and the. The present crosssectional survey compared the diagnostic performance of the kk technique and the poccca test in. Comparison of the kato katz thick smear and mcmaster egg counting techniques for monitoring drug efficacy against soiltransmitted helminths in schoolchildren on pemba island, tanzania.
Sandwich or teesdale technique, which is very similar to the katokatz technique. For the other sths, the new method found the same prevalence as the kato katz technique. Useful as it is, the technique failed to preserve hookworm eggs in the slide after 2 hours. We assessed the costs related to the collection, processing. An optimum therapeutic dose range of 1530 mgkg oxantel pamoate was defined. The kato katz technique is recommended for the diagnosis of helminth infections in epidemiological surveys, drug efficacy studies and monitoring of control interventions. Author summary currently, in public health, the reduction in the number of eggs excreted in stools after drug administration is used to monitor the efficacy of drugs against parasitic worms. We compared the kato katz thick smear kato katz and mcmaster egg counting mcmaster methods, which are commonly. Comparative study of the accuracy of different techniques. Comparison of the katokatz thick smear and mcmaster egg. Kato katz technique is used for qualitative and semiquantitative diagnosis of intestinal helminthic infestations. The epg values are used as a proxy for infection intensity.
In this paper, we compared the standard kato katz kk technique with a traditional fixation. Five grams of fresh feces were weighed and homogenized by strong manual agitation in a 50ml capped plastic tube containing 25 ml of 0. The kato katz technique is a simple, practical, and helpful tool used to diagnose infection with soil transmitted helminthes. In addition to extensive coverage of aspects of technique, the book addresses note reading, musical form, relationships between repertoire and scales, practicing. Rabello, comparison of a polymerase chain reaction and the kato katz technique for diagnosing infection with schistosoma mansoni, the american journal of tropical. It has in the past been used for other helminth eggs as well. Of note, kato katz thick smears are usually examined for concurrent soiltransmitted helminth eggs. Statistical analysis of the results showed a kappa index of. With a weight independent dose of 500 mg oxantel pamoate 95% of children aged 714 years in subsaharan africa would receive doses of 117320 mgkg. The kato katz technique is widely used for the diagnosis of soiltransmitted helminthiasis in epidemiological surveys and is believed to be an inexpensive method. Where the prevalence of sth is technique makes it less appropriate, and more sensitive tools should be used. A crosssectional study on schistosomiasis and soil.
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